A three-allele restriction-fragment-length polymorphism at the hypoxanthine phosphoribosyltransferase locus in man.
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چکیده
منابع مشابه
Rapid communication: a MspI restriction fragment length polymorphism at the swine PIT-1 locus.
Name of Marker. Swine PIT-1*2. Source and Description of Clone. Probe used in RFLP analysis was a cDNA fragment encoding the swine PIT-1 POU-domain region (Tuggle et al., 1993). The 311-bp probe was isolated from the vector pCRlOOO using EcoRI and HindIII.
متن کاملRestriction Fragment Length Polymorphism (RFLP)
Restriction Fragment Length Polymorphism (RFLP) is a technique in which organisms may be differentiated by analysis of patterns derived from cleavage of their DNA. If two organisms differ in the distance between sites of cleavage of a particular restriction endonuclease, the length of the fragments produced will differ when the DNA is digested with a restriction enzyme. The similarity of the pa...
متن کاملRapid communication: StuI restriction fragment length polymorphism at the porcine bone morphogenetic protein 5 (BMP5) locus.
Source and Description of Probe. A 2.2-kb human cDNA clone for bone morphogenetic protein 5 (BMP5) was excised from the clone U2-16 (Celeste et al., 1990).
متن کاملHgi AI restriction fragment length polymorphism at the human glutathione S-transferase 2 locus.
SOURCE/DESCRIPTION: A plasmid containing a 700 bp cDNA fragment (pGST2-Pvu II) was used as a probe (1). POLYMORPHISM: Hgi AI (GJGCJ/C) identifies a two allele polymorphism with bands at about 8.1 kb and 7.2 kb. CHROMOSOMAL LOCALISATION: The GST-2 gene has been localized on the short arm of human chromosome 6 (1).
متن کاملRapid communication: MspI restriction fragment length polymorphism at the swine myogenin locus.
Source and Description of Probe. A 1,486-bp rat cDNA clone for myogenin (MYOG) was excised from the EcoRI site of the plasmid, pBluescrybe M13(Wright et al., 1989). Method of Detection. DNA was isolated from whole blood and digested with MspI. Fragments were then separated by agarose gel electrophoresis and transferred to charged nylon membranes. Hybridizations were at 65°C for 16 to 20 h (.5 M...
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ژورنال
عنوان ژورنال: Proceedings of the National Academy of Sciences
سال: 1983
ISSN: 0027-8424,1091-6490
DOI: 10.1073/pnas.80.13.4035